Two Frontiers Project INC EIN 99-2011215

Two Frontiers Project INC

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Exploring, understanding, and harnessing microbial life from sea to space. For fiscal year 2024 it reported $538K in revenue, $256K in expenses, and $282K in net assets.Pt I

Type
Public charity (501(c)(3)) · Science & Technology
Location
Fort Collins, CO
Website
www.twofrontiers.org
Filings
1 on file (2024–2024)
Revenue
$538KFY2024
Expenses
$256K
Net assets
$282K
People
4
Filings
1
Updates
0
More identity details & actions ⌄
EIN  99-2011215 Public charity (501(c)(3)) Fort Collins, CO
Form 990 (PDF)
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Size
$100K–1M
What they do
Exploring, understanding, and harnessing microbial life from sea to space.
Leadership
Krista Ryon · Treasurer and Director of Operations · $4K
Money in and out
$538K revenue, $256K expenses
Bottom line
91% program efficiency
Where the money goes · FY2024
Total revenue
$538K
Pt VIII · Ln 12
Total expenses
$256K
Pt IX · Ln 25
Net assets
$282K
Pt X · Ln 32
Revenue less expenses (Pt I · Ln 19): $283K
Where spending went · Part IX cols B–D
Program services $0.91 Management & general $0.08 Fundraising $0.01
Program efficiency
91%
of spending reaches programs
Operating runway
24.0mo
months of highly liquid reserves at operating expense rate
Surplus margin
+52%
revenue over expenses, this year

Summary of the Filing

Part I · fiscal year 2024 · Form 990
Total revenue
$538K
Pt VIII · Ln 12
Total expenses
$256K
Pt IX · Ln 25
Net assets
$282K
Pt X · Ln 32
Revenue less expensesPt I · Ln 19$283K
Total assetsPt X · Ln 16$286K
Program-expense ratioPt IX · col B91%
Voting members of governing bodyPt I · Ln 34
Independent voting membersPt I · Ln 42
Mission & Programs · Part III

Where the work happens

3 program services account for $228K of program spending, described in the organization's own filed words · FY2024.
01

In 2024, the two frontiers project advanced its carbon initiative, focused on discovering and characterizing novel microorganisms that naturally capture and recycle carbon. The team conducted fieldwork in japan and papua new guinea, isolating and analyzing cyanobacteria such as the newly identified chonkus lineage capable of enhanced…

$114Kprogram expense
02

Through its crops initiative, the two frontiers project began building a research platform to identify and culture microorganisms that enhance agricultural productivity, water retention, and plant resilience under drought and nutrient stress.

$106Kprogram expense
03

The coral initiative focused on the development of open-source bioinformatic software and workflows for analyzing coral-associated microbial communities. The organization advanced two flagship tools magus (HTTPS://GITHUB.COM/TWO-FRONTIERS-PROJECT/2FP_MAGUS, for metagenomic assembly and binning) and xtree…

$7Kprogram expense

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Identity & Classification

Form 990 Header · IRS Business Master File
Legal nameHdr · item CTwo Frontiers Project INC
EINHdr · item D99-2011215
Principal addressHdr · item CFort Collins, CO
WebsiteHdr · item Jwww.twofrontiers.org
Year of formationHdr · item Lpending
State of legal domicileHdr · item MCO
Exempt statusHdr · item I501(c)(3) · active
NTEE classificationIRS BMFScience & Technology (U05)
Ruling yearIRS BMFAug 2024

Governance & Policies

Part VI
Voting members of the governing bodyPt VI · Ln 1a4
Independent voting membersPt VI · Ln 1b2
Conflict-of-interest policyPt VI · Ln 12aYes
Whistleblower policyPt VI · Ln 13No
Document retention policyPt VI · Ln 14No
CEO/top-official compensation independently reviewedPt VI · Ln 15aNo
Family or business relationships among officers/directorsPt VI · Ln 2No

Officers, Directors & Key Employees

Part VII, Section A · reportable compensation
Treasurer and Director of Operations
$4,263Pt VII · Sec A
President and Executive Director
$0Pt VII · Sec A
Secretary and Director of Research and Development
$0Pt VII · Sec A
Director
$0Pt VII · Sec A

Krista Ryon’s $4K as Treasurer and Director of Operations is at the 4th percentile of top reported officer pay among 310 $100K–1M science & technology nonprofits. Peer set: organizations in the same NTEE major field of work and revenue-size band, compared on each org’s own highest-paid officer/key-employee, from Form 990 Part VII / 990-PF Part VIII. Descriptive placement, not a verdict on whether the pay is appropriate.

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Statement of Revenue

Part VIII
Contributions & grants100%$538K
Other revenue$158
Total revenueLn 12$538K

Statement of Functional Expenses

Part IX · allocated across program, management, and fundraising
Allocation · columns B–D
Program services $0.91 Management & general $0.08 Fundraising $0.01
Program services91%$233K
Management & general8%$22K
Fundraising1%$2K
Total functional expensesLn 25$256K

Balance Sheet

Part X · end of year
CashLn 1$265K
Total assetsLn 16$286K
Total liabilitiesLn 26$3K
Total net assetsLn 32$282K
Months of cash on handcomputed12.4

Financial Metrics

Form 990 · FY2024 · each ratio shown with the dollars behind it
Liquidity & Payout
Operating runway
Highly liquid reserves $265K · Operating expenses (Pt IX) $256K · Less non-cash grants $123K · Cash operating expenses/yr $132K
24.0 months
Where the money goes
Program services
Program services $233K · Total expenses $256K
91%
Management & General
Management & general $22K · Total expenses $256K
8%
Fundraising
Fundraising $2K · Total expenses $256K
0.6%
Cost to raise $1
Fundraising expense $2K · Solicited contributions $538K
$0.00 to raise $1
Where the money comes from
Contribution dependence
Contributions & grants $538K · Total revenue $538K
100%
Government reliance
Government grants — · Total revenue $538K
Earned-income share
Program service revenue $0 · Total revenue $538K
0%
Investment reliance
Investment income $0 · Total revenue $538K
0%
Program self-sufficiency
Program service revenue $0 · Total expenses $256K
0%
Growth & trend
Revenue growth (YoY)
This year $538K · Prior year —
No prior year on file
Net-asset trend (YoY)
End of year $282K · Beginning of year —
Beginning net assets not meaningfully positive — showing dollar change
Balance sheet
Surplus Margin
Revenue $538K · Expenses $256K
+52%
Liabilities-to-Assets
Total liabilities — · Total assets $286K
Net-asset ratio
Net assets $282K · Total assets $286K
99%
Marketable Investment Share
Publicly traded securities (Pt X, ln 11) — · Other securities (Pt X, ln 12) — · Total assets (Pt X, ln 16) $286K
Excludes program-related investments (Pt X, ln 13)
People & payroll
Highest Reported Total Compensation
Individual Krista Ryon · Reported title TREASURER AND DIRECTOR OF OPERATIONS · Highest reported compensation $4K · Total expenses $256K
2%
Personnel share
Salaries, benefits & payroll (Pt IX 5-10) $13K · Total expenses $256K
5%
Peer comparison

Same NTEE category, revenue band, and state where available. Descriptive — not a grade.

MetricThis orgPeer medianPercentile
Program ratio 91%
Overhead ratio 8%
Fundraising cost ratio 0.3%
Legal fee ratio 0.2%
Accounting fee ratio 3%

Straight from the Form 990 — descriptive, not an evaluation. A ratio is hidden when its base is zero or too small to be meaningful; the dollars are always shown.

Grants

Schedule I · received and paid
Grants received · FY2024
Total grants receivedfrom 2 funders$404K
Grants paid · 1 grant · $50K · 2024–2024
Colorado State UniversityFY2024$50,000

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Filings & Schedule Manifest

Part IV
III · Programs I · Grants R · Related orgs G · Fundraising L · Interested persons A · Public support F · Foreign M · Noncash
FY2024 (Latest)$538K revenue · viewing · ⤓ 990 PDF

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Sources

Every figure above is drawn from these filings
Form 990 e-file (XML) · FY2024IRS
Classification, formation year, addressIRS Business Master File
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